Hayat cetvelinin diğer uç kısmında ise Paris japonica bitkisi var. Zambak ailesinin üyesi olan bitkinin kalıtımı insanınkine oranla aşağı yukarı 50 kat daha büyük durumda. Ayrıca yaklaşık olarak 22.000 genden meydana geliyor. Doğal canlı sınıfı arasında “minimalist yaşam” rekorunu, 525 gen ile Mycoplasma genitalium bakterisi sahipleniyor.
Venter ile çalışmayı sürdüren ekip doğrulusunda imal edilmiş olan “Syn 3.0” artık bu bakterinin rekorunu egale etmiş oldu. Adından da anlaşıldığı gibi Syn 3.0 sentetik olarak imal edilen ilk canlı değil. Venter ile çalışan incelemeciler 2010 senesinde bir bakterinin tek bir kalıtımsal bir bölümünü üretmişlerdi. Syn 1.0 olarak adlandırılan bu hücre sadece üstünde depolanmış maddeleri üretebiliyordu.
Akıllarda Sorular Başını Tam Alıyorken...
Yapılan en son çalışmadaki soru ise şu şekildeydi: Yapay bir bakterinin hayatını devam ettirebilmesi ve bölünebilmesi maksadıyla Syn 1.0’deki kaç gen yeterli olabilir? Baştaki deneyde incelemeciler kalıtım ile ilgili bütün bilgilerden yararlanarak, masa başında bu tür bir minimal organizma tasarlamaya çalıştılarsa da başarılı olunamamıştır. Halihazırda ki biyoloji verimiz yaşam sürdüren bir organizma için tasarlanıp, hücreleri üretmek amacıyla yeterli olmadı. Başarıya giden yol “deneme” ve “ hata” olduğunu Venter belirtiyor.
Yetkililer Syn 1.0’ın 901 genden oluşan kalıtımını; sekiz parçaya böldükten sonra genleri ayırmış ve sonuçları Mycoplasma capricolum bakterisine entagrasyon sağlamışlardır. Neredeyse tüm vakalarda hiçbir sonuca gidilememiştir. Minimal DNA’lı yapay hücreler hayatını devam ettirememiştir. Fakat birkaç denemenin ardından araştırmacılar sonunda Syn 3.0 olarak isimlendirdikleri varyantı elde edebilmişlerdir.
Sentetik bakterinin kalıtımı yarı yarıya küçük olmasına karşın, neredeyse yaşam için gerekli tüm işlevlere sahip durumda. Tabi diğer yandan bakteride protein maddeleri üretilmesinden sorumlu genlerin çoğunluğa yakınının eksik ama bilgi aktarımı ve ekspresyon için gerekli genlerin çoğu var olduğu da gözlemlenmiştir. Bununla beraber 473 genin tam olarak neleri yerine getirdiklerini; Venter’ın ekibi henüz açıklayamıyor. Genlerin halen üçte birinin işlevi tam anlamıyla bilinmiyor.
Minimal genli organizmalarla araştırmanın daha kolay olması sebebiyle (örnek olarak bazı yeni genler ilave edilerek nelerin yaşandığı gözlemlenip, işlevlerinin ne olduğu bilinebilir) araştırmacılar önemli bir buluş yaptıklarının farkında olmanın haklı gururunu yaşamaya devam ediyorlar.
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